Insulin aspart ®


Novo Nordisk

Insulin aspart (marketed by Novo Nordisk as “NovoLog/NovoRapid”) is a fast acting insulin analogue. It was created through recombinant DNAproline, is substituted with an aspartic acid residue. This analogue has increased charge repulsion, which prevents the formation of hexamers, to create a faster acting insulin. The sequence was inserted into the yeast genome, and the yeast expressed the insulin analogue, which was then harvested from a bioreactor.

The components of insulin aspart are as follows: Metal ion – zinc (19.6 ug/mL) Buffer – disodium hydrogen phosphate dihydrate (1.25 mg/mL) Preservative – m-cresol (1.72 mg/mL) and phenol (1.50 mg/mL) Isotonicity agent – glycerin (16 mg/mL) and sodium chloride (0.58 mg/mL). The pH of insulin aspart is 7.2-7.6.
According to JDRF, insulin aspart was approved for marketing in the United States by the Food and Drug Administration in June 2000.
Can be used in CSII pumps and Flexpen, Novopen delivery devices for subcutaneous injection. Additionally, it can be used with an injection port such as the I-port.
The onset of action is approximately 15 minutes, the peak action is reached in 45-90 minutes, and the duration is 3-5 hours. However, it should be noted that as with all insulin, these numbers are based on averages, and vary between individuals due to blood flow, injection site, temperature and exercise.
It has been debated whether or not insulin aspart (or NovoLog/NovoRapid) should be refrigerated. Recent studies have shown that there is no difference, however insulin should not be stored in intense heat for a long period of time. This causes the hormone to reach a pre- boiling state, and separate. This can change the potency of the insulin, and it would no longer be as effective. A good rule of thumb is to keep the insulin at around room temperature or less.
technology so that the amino acid, B28, which is normally

Variations on Insulin Aspart

NovoLog Mix 70/30 is a product marketed by Novo Nordisk which contains 30% insulin aspart and 70% insulin aspart protamine. The insulin aspart protamine portion is a crystalline form of insulin aspart, which delays the action of the insulin, giving it a prolonged absorption profile after injection. The combination of the fast-acting form and the long-acting form allows the patient to receive fewer injections over the course of the day.

The components of NovoLog Mix 70/30 are as follows: Metal ion – zinc (19.6 ug/mL) Buffer – dibasic sodium phosphate (1.25 mg/mL) Preservative – m-cresol (1.72 mg/mL) and phenol (1.50 mg/mL) Isotonicity agent – sodium chloride (0.58 mg/mL) and mannitol (36.4 mg/mL) Modifying protein – protamine (0.33 mg/mL) The pH is 7.2-7.44
NovoLog Mix is marketed to be used with the Novo Nordisk FlexPen The onset of action is less than 30 minutes, the peak action is reached in 1-4 hours, and the duration is less than 24 hours.